Purring Tiger's
Classroom Critters

 PARAMECIUM

SCIENTIFIC NAME
Paramecium aurelia; Paramecium caudatum

GENERAL
Paramecium are oval-shaped organisms with their surface covered with cilia. They rotate as they travel forward through the water.

REPRODUCTION
Asexually by transverse division or sexually by conjugation. Paramecium aurelia may divide 5 times a day while Paramecium caudatum may divide only 3 times.

CARE

CULTURE VESSEL
Any clean glass container with a cover to protect from dust will do. Clean the container and all transfer droppers with hot water and 70% rubbing alcohol. Rinse thoroughly in clean water.

TEMPERATURE (F)
Water should be 68-76 degrees.

pH
Neutral to slightly alkaline.

LIGHT
Normal lighting conditions without direct light and no sunlight.

CULTURE MEDIUM
Use boiled spring water or boiled aquarium water in each of the following methods: 1) Pour 200 mL water into a culture dish and add 8 boiled wheat grains. Allow to cool for 6 hours and add paramecium. Subdivide the culture every 2-4 weeks to prevent overcrowding. 2) Add one Carolina Biological Protozoan Pellet (item #13-2360 Protozoan Pellet from www.carolina.com) to 1000 mL of water and allow the pellet to disintegrate (Make sure that the water has cooled BEFORE adding the pellet). Divide the liquid and residue into 150-200 mL amounts in each of several culture dishes. Add the paramecium and 2-4 boiled grains. Subdivide the culture every 2-4 weeks to prevent overcrowding.

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